Shinwa Chemical Industries Ltd.
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Shinwa Chemical Industries
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"Phosphorothioate oligo-deoxythymidine" Filtered 6 Item.

Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 1) “Relationship between PS site and retention time in dT6”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 25/75
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 2) “Relationship between number of PS modification and retention time in dT6”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 25/75
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

View detail


Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 3) “Relationship between PS site and retention time in phosphorothioate-modified dT6”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 23/77
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

View detail


Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 4) “Relationship between PS sites and retention time in dT20”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 38/62
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

View detail


Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 1) “Effect of salt concentration in mobile phase”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100-300 mM CH3CO2NH4/CH3CN = 35/65
Frow rate
0.15 mL/min
Column temp.
40°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

View detail


Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 2) “Effect of analysis temperature”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
200 mM CH3CO2NH4/CH3CN = 35/65
Frow rate
0.15 mL/min
Column temp.
10-50°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

View detail