LCSamplesPhosphorothioate oligo-deoxythymidineApplicationsAnalysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 1) “Relationship between PS site and retention time in dT6”ColumnULTRON AF-HILIC-CDColumn Size150 mm L × 2.0 mm I.D.Mobile phase100 mM CH3CO2NH4/CH3CN = 25/75Frow rate0.15 mL/minColumn temp.30°CInjection volume0.5 µLDiluentCH3CN/TE buffer※ = 70/30 View detail
LCSamplesPhosphorothioate oligo-deoxythymidineApplicationsAnalysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 2) “Relationship between number of PS modification and retention time in dT6”ColumnULTRON AF-HILIC-CDColumn Size150 mm L × 2.0 mm I.D.Mobile phase100 mM CH3CO2NH4/CH3CN = 25/75Frow rate0.15 mL/minColumn temp.30°CInjection volume0.5 µLDiluentCH3CN/TE buffer※ = 70/30 View detail
LCSamplesPhosphorothioate oligo-deoxythymidineApplicationsAnalysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 3) “Relationship between PS site and retention time in phosphorothioate-modified dT6”ColumnULTRON AF-HILIC-CDColumn Size150 mm L × 2.0 mm I.D.Mobile phase100 mM CH3CO2NH4/CH3CN = 23/77Frow rate0.15 mL/minColumn temp.30°CInjection volume0.5 µLDiluentCH3CN/TE buffer※ = 70/30 View detail
LCSamplesPhosphorothioate oligo-deoxythymidineApplicationsAnalysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 4) “Relationship between PS sites and retention time in dT20”ColumnULTRON AF-HILIC-CDColumn Size150 mm L × 2.0 mm I.D.Mobile phase100 mM CH3CO2NH4/CH3CN = 38/62Frow rate0.15 mL/minColumn temp.30°CInjection volume0.5 µLDiluentCH3CN/TE buffer※ = 70/30 View detail
LCSamplesPhosphorothioate oligo-deoxythymidineApplicationsAnalysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 1) “Effect of salt concentration in mobile phase”ColumnULTRON AF-HILIC-CDColumn Size150 mm L × 2.0 mm I.D.Mobile phase100-300 mM CH3CO2NH4/CH3CN = 35/65Frow rate0.15 mL/minColumn temp.40°CInjection volume0.5 µLDiluentCH3CN/TE buffer※ = 70/30 View detail
LCSamplesPhosphorothioate oligo-deoxythymidineApplicationsAnalysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 2) “Effect of analysis temperature”ColumnULTRON AF-HILIC-CDColumn Size150 mm L × 2.0 mm I.D.Mobile phase200 mM CH3CO2NH4/CH3CN = 35/65Frow rate0.15 mL/minColumn temp.10-50°CInjection volume0.5 µLDiluentCH3CN/TE buffer※ = 70/30 View detail