Shinwa Chemical Industries Ltd.
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Shinwa Chemical Industries
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"Thymidine" Filtered 11 Item.

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LC

Samples
Cytosine , Uracil , Guanine , Adenine , Cytidine , Uridine , Thymine , Adenosine , Guanosine , Thymidine
Applications
Nucleic acid base, Nucleoside
Column
STR ODS-II
Column Size
150 mm L × 4.6 mm I.D.
Mobile phase
0.1 M Phosphoric acid buffer (pH 2.1) + 0.2 M NaClO4
Frow rate
1.0 mL/min
Column temp.
50°C

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LC

Samples
Toluene , Uridine , 2'-Deoxyuridine , 5-Methyluridine , Adenosine , Vidarabine , 2'-Deoxyguanosine , 3'-Deoxyguanosine , 4-Nitrophenyl-α-glucopyranoside , 4-Nitrophenyl-β-glucopyranoside , Theophylline , Theobromine ,
Applications
Analysis of polar compounds
Column
ULTRON AF-HILIC-CD
Column Size
100 mm L × 4.6 mm I.D.
Mobile phase
20mM CH3CO2NH4 (pH4.7) /CH3CN = 10/90
Frow rate
0.50 mL/min
Column temp.
30°C
Injection volume
4.0 µL
Diluent
mobile phase

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LC

Samples
Toluene , Uridine , 2'-Deoxyuridine , 5-Methyluridine , Adenosine , Vidarabine , 2'-Deoxyguanosine , 3'-Deoxyguanosine , 4-Nitrophenyl-α-glucopyranoside , 4-Nitrophenyl-β-glucopyranoside , Theophylline , Theobromine ,
Applications
Analysis of polar compounds
Column
ULTRON AF-HILIC-DA
Column Size
100 mm L × 4.6 mm I.D.
Mobile phase
20mM CH3CO2NH4 (pH4.7) /CH3CN = 10/90
Frow rate
0.50 mL/min
Column temp.
30°C
Injection volume
4.0 µL
Diluent
mobile phase

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LC

Samples
Oligo deoxythymidine primers , Oligo dT primers , Oligo deoxythymidine, 6-25 mer
Applications
Analyses of Oligo deoxythymidine primers
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
A: CH3CN B: 100 mM CH3CO2NH4 (pH 6.7)
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
5.0 µL
Diluent
CH3CN/TE buffer* = 70/30

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LC

Samples
Oligo deoxythymidine primers, 6-25 mer
Applications
Analysis of Oligo-deoxythymidine using the ULTRON AF-HILIC-CD “Effect of the type of salt and ionic strength in the mobile phase”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer* = 70/30

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Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 1) “Relationship between PS site and retention time in dT6”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 25/75
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 2) “Relationship between number of PS modification and retention time in dT6”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 25/75
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 3) “Relationship between PS site and retention time in phosphorothioate-modified dT6”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 23/77
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of Phosphorothioate oligo-deoxythymidine using the ULTRON AF-HILIC-CD (Part 4) “Relationship between PS sites and retention time in dT20”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100 mM CH3CO2NH4/CH3CN = 38/62
Frow rate
0.15 mL/min
Column temp.
30°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 1) “Effect of salt concentration in mobile phase”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
100-300 mM CH3CO2NH4/CH3CN = 35/65
Frow rate
0.15 mL/min
Column temp.
40°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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Graph

LC

Samples
Phosphorothioate oligo-deoxythymidine
Applications
Analysis of PSdT6 and [PHO]-PSdT6-[PHO] using the ULTRON AF-HILIC-CD (Part 2) “Effect of analysis temperature”
Column
ULTRON AF-HILIC-CD
Column Size
150 mm L × 2.0 mm I.D.
Mobile phase
200 mM CH3CO2NH4/CH3CN = 35/65
Frow rate
0.15 mL/min
Column temp.
10-50°C
Injection volume
0.5 µL
Diluent
CH3CN/TE buffer※ = 70/30

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