LC
- Samples
- (CYS)2 , LYS , ARG , HIS , ASP , GLU , GLN , ASN , GLY , SER , ALA , THR , PRO , TYR , VAL , PHE , MET , TRP , LEU+ILE
- Applications
- Analysis of underivatized amino acids by LC/MS/MS
- Column
- ULTRON AF-HILIC-CD (PLSS)
- Column Size
- 150 mm L × 2.0 mm I.D.
- Detector
- LC/MS/MS (ESI, Positive, MRM)
- Particle Size
- 2 µm
- Mobile phase
- A: 100 mM HCO2NH4(pH 3.0)/CH3CN = 10/90, B: 100 mM HCO2NH4(pH 3.0)/CH3CN = 70/30
- Frow rate
- 0.36 mL/min
- Gradient
- 20 minutes gradient, 88% - 60% with 10 minutes re-equilibration
Time Profile (min) %A %B 0.00 96 4 20.00 50 50 20.01 96 4 30.00 96 4 - Column temp.
- 40°C
- Injection volume
- 1.0 µL (5.0 µg/mL)