LC
- Samples
- Acesulfame K , Saccharin , Caffeine , Sorbic acid , Benzoic acid , Quinine , Aspartame
- Applications
- Analysis of food additives
- Column
- ULTRON AF-HILIC-CD
- Column Size
- 150 mm L × 3.0 mm I.D.
- Detector
- UV-254 nm
- Particle Size
- 2 µm
- Mobile phase
- A: 20mM CH3CO2NH4 (pH4.7) /CH3CN = 10/90, B: 20mM CH3CO2NH4 (pH4.7) /CH3CN = 70/30
- Frow rate
- Refer to the picture
- Gradient
- 10 minutes gradient, 90% - 60% with 20 minutes re-equilibration
Time Profile (min) %A %B 0.00 100 0 10.00 50 50 10.01 100 0 12.00 100 0 23.00 100 0 25.00 100 0 30.00 100 0 - Column temp.
- 30°C
- Injection volume
- 2.0 µL
- Diluent
- H2O /CH3CN = 50/50